Microsatellite Analysis To Identify Resistance of Giant Gouramy (Osphronemus gouramy Lac.) To Aeromonas hydrophila
Meirina Kartika Kusumawardhani, Diah Kusumawaty and Sony Suhandono 2014

Giant gouramy is one of freshwater fish with the highest demand, but the production was declined because of a Motile Aeromonas Septicemia (MAS) disease. Giant gouramy with MAS disease will have an ulcer on their skin, and worst infection will cause death. The symptoms is vary widely depends on fish resistance. The aim of this study is to scanning the resistance of giant gouramy broodstock from Tasikmalaya, Singaparna, and Sukabumi against Aeromonas hydrophila by microsatellite analysis method. Three informative loci with polymorphic information content (PIC) value 0.65, 0.62, and 0.46 was selected from eleven microsatellite loci. Forward primer was added with M13 sequences that complement with a fluorescence dye. Amplification of the microsatellite loci performed by PCR on DNA samples of resistant and sensitive giant gouramy. Successfully amplified alleles were analyzed by calculating the genetic distance (GD) and visualized by principal coordinate analysis (PCoA). The result shows that 56.25% giant gouramy broodstock have close to resistance giant gouramy. It means aproximately half of giant gouramy broodstock from Tasikmalaya, Singaparna, and Sukabumi suspected to be resistant against Aeromonas hydrophila.