Isolasi dan Karakterisasi Gen Penyandi Protein Permukaan VP28 White Spot Syndrome Virus (WSSV) pada Udang Windu (Penaeus monodon Fabricius, 1798)
White Spot Syndrome (WSS) is a viral disease which affects most of the commercially cultivated marine shrimp species all over the world causing significant losses. White spot syndrome virus (WSSV) envelope protein VP28 gene is widely used because its ability to bind to the surface of shrimp epithelial cells and might promote innate immune recognition of WSSV. Its recombinant protein was expressed in various expression systems and used as recombinant vaccine or immunostimulant to increase shrimp survival against WSSV. This research was aimed to isolate and characterize gene encoding envelope protein VP28 WSSV from black tiger shrimps (Penaeus monodon Fabr). The genomic of DNA were isolated from pleopods, periopods and tails of black tiger shrimp using DTAB-CTAB method. Isolation of gene encoding envelope protein VP28 WSSV ws successfully performed with the results of the length of DNA fragment was 672 bp. The results of homology analysis using BLASTn homology suggested that these isolates genes from Takalar have closest relationship with isolates from India.